Publications | Centre for Tuberculosis Research

Selected recent publications directly related to tuberculosis research:

2014
Casabon I, Swain K, Crowe AM, Eltis LD, Mohn WW. 2014. Actinobacterial acyl-CoA synthetases involved in steroid side chain catabolism. J. Bacteriol. 196, 579-87.

2013
Casabon I, Crowe AM, Liu J, Eltis LD. 2013. FadD3, an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. Mol. Microbiol. 87, 269-83.

Casabon I, Zhu S, Otani H, Liu J, Mohn WW, Eltis LD. 2013. Regulation of the KstR2 regulon of Mycobacterium tuberculosis by a cholesterol catabolite. Mol. Microbiol. 89, 1201-12.

Farhat MR, Shapiro BJ, Kieser KJ, Sultana R, Jacobson KR, Victor TC, Warren RM, Streicher EM, Calver A, Sloutsky A, Kaur D, Posey JE, Plikaytis B, Oggioni MR, Gardy JL, Johnston JC, Rodrigues M, Tang PK, Kato-Maeda M, Borowsky ML, Muddukrishna B, Kreiswirth BN, Kurepina N, Galagan J, Gagneux S, Birren B, Rubin EJ, Lander ES, Sabeti PC, Murray M. 2013. Genomic analysis identifies targets of convergent positive selection in drug-resistant Mycobacterium tuberculosis. Nat Genet.45(10):1183-9.

Ford CB, Shah RR, Maeda MK, Gagneux S, Murray MB, Cohen T, Johnston JC, Gardy JL, Lipsitch M, Fortune SM. 2013. Mycobacterium tuberculosis mutation rate estimates from different lineages predict substantial differences in the emergence of drug-resistant tuberculosis. Nat Genet. 45(7):784-90.

Gardy JL.. 2013. Investigation of disease outbreaks with genome sequencing. Lancet Infect Dis. 2013 (2):101-2.

Gómez-Velasco, A., Bach, H., Rana, K. A., Cox, R.L., Bhatt, A., Besra, S, G., and Av-Gay. Y.2013. Disruption of the serine threonine protein kinase H affects phthiocerol dimycocerosates synthesis in Mycobacterium tuberculosis. Microbiology. 159:726-36.

Liu X, Song F, Ma L, Chen C, Xiao X, Ren B, Liu X, Dai H, Piggott A, M., Av-Gay, Y, Zhang L and Capon, R J. Sydowiols A-C. 2013. Mycobacterium tuberculosis protein tyrosine phosphatase inhibitors from an East China sea marine-derived fungus, Aspergillus sydowii. Tetrahedron Letters, 54 45: 6081-6083.

Sun J, Singh V, Lau A, Stokes RW, Obregón-Henao A, Orme IM, Wong D, Av-Gay Y, Hmama Z.2013. Mycobacterium tuberculosis nucleoside diphosphate kinase inactivates small GTPases leading to evasion of innate immunity. PLoS Pathog. Jul 9 (7):e1003499.

Wong , D., Chao, J.D., and Av-Gay, Y . Mycobacterium tuberculosis-secreted phosphatases: from pathogenesis to targets for TB drug development. 2013. Trends in Microbiology. 21:100-109

2012
Burian J., Ramón-García S., Howes CG., Thompson CJ. (2012) “WhiB7, a transcriptional activator that coordinates physiology with intrinsic drug resistance in Mycobacterium tuberculosis”. Expert Review of Anti-infective Therapy. Vol. 10, No. 9, p. 1037-1047 (doi: 10.1586/eri.12.90).

Burian J., Ramón-García S., Sweet G., Gómez-Velasco A., Av-Gay Y., and Thompson C.J. (2012) “The mycobacterial transcriptional regulator whiB7 links redox homeostasis and intrinsic antibiotic resistance.” Journal of Biological Chemistry. 287:299-310. Epub 2011 Nov 8.

Capyk JK, Eltis LD. 2012. Phylogenetic analysis reveals the surprising diversity of an oxygenase class. J. Biol. Inorg. Chem. 17, 425-36.

Capyk JK, Casabon I, Gruninger R, Strynadka NC, Eltis LD. 2011. The substrate specificity of KshAB indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis. J. Biol. Chem. 286, 40717-24.

Casabon I, Crowe AM, Liu J, Eltis LD. 2012. FadD3, an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. Mol. Microbiol., In press.

Centko, RM., Ramón-García, S., Taylor, T., Patrick, BO., Thompson, CJ, Miao, VP., Andersen, RJ. (2012) “Ramariolides A-D, Antimycobacterial Butenolides Isolated from the Mushroom Ramaria cystidiophora”. dx.doi.org/10.1021/np3006277 | J. Nat. Prod.

Cook VJ, Shah L, Gardy JL. Modern contact investigation methods for enhancing tuberculosis control in aboriginal communities. Int J Circumpolar Health. 2012. 25:18643.

Cook VJ, Shah L, Gardy JL, Bourgeois AC. Recommendations on modern contact investigation methods for enhancing tuberculosis control. Int J Tuberc Lung Dis. 2012. 16:297-305.

Lam KK, Zheng X, Forestieri R, Balgi AD, Nodwell M, Vollett S, Anderson HJ, Andersen RJ, Av-Gay Y. , Roberge M. Nitazoxanide stimulates autophagy and inhibits mTORC1 signaling and intracellular proliferation of Mycobacterium tuberculosis. 2012. PLoS Pathog. May 8(5):e1002691.

Lim, L., Vilchèze, C., Ng C., Jacobs, W., Ramón-García, S.*,†, and Thompson, CJ.† (2012) “Anthelmintic avermectins kill M. tuberculosis, including multidrug resistant clinical strains”. Antimicrob. Agents Chemother. AAC Accepts, published online ahead of print on 19 November 2012 doi:10.1128/AAC.01696-12.

Mohn WW, Wilbrink MH, Casabon I, Stewart G, Liu J, van der Geize R, Eltis LD. 2012. A gene cluster encoding bile acid metabolism in Rhodococcus spp. J. Bacteriol. 194, 6712-6719.

Poirier V, Av-Gay Y., Mycobacterium tuberculosis modulators of the macrophage’s cellular events.2012. Microbes Infect. 13:1211-1219. PMID: 22841679

Ramón-García S.*, Mick V., Dainese E., Martín C., Thompson C.J., De Rossi E., Manganelli R., and Aínsa J.A. (2012) “Functional and genetic characterization of the Tap efflux pump in Mycobacterium bovis BCG.” Antimicrobial Agents & Chemotherapy. 56: 2074-2083. Epub 2012 Jan 9.

Swain K, Casabon I, Eltis LD, Mohn WW. 2012. Two transporters essential for the reassimilation of novel cholate metabolites by Rhodococcus jostii RHA1. J. Bacteriol. 194, 6720-6727.

2011

Bach SM, Fortuna MA, Attarian R, de Trimarco JT, Catalán CA, Av-Gay Y, Bach H. Antibacterial and cytotoxic activities of the sesquiterpene lactones cnicin and onopordopicrin. Nat Prod Commun. 2011 Feb;6(2):163-6.

Gardy, JL., Johnston, JC, Shannan J. Ho Sui, Victoria J. Cook, Shah, L, Brodkin, E, Rempel, S, Moore, R, Zhao, Y, Holt, R, Varhol, R, Birol, I, Lem, M, Sharma, MK, Elwood, K, Jones, SJM, Brinkman, FSL, Brunham, RC and Tang, P. Whole-Genome Sequencing and Social-Network Analysis of a Tuberculosis Outbreak. N. Engl. J Med. 364:730-9.

Ramón-García S, Ng C, Anderson H, Chao JD, Zheng X, Pfeifer T, Av-Gay Y , Roberge M, Thompson CJ. Synergistic drug combinations for tuberculosis therapy identified by a novel high throughput screen. Antimicrob Agents Chemother. 16 May. PMID: 21576426.

Xu X, Vilcheze C, Av-Gay Y, Gómez-Velasco A, Jacobs WR Jr. Precise null deletion mutations of the mycothiol synthetic genes reveal their role in isoniazid and ethionamide resistance in Mycobacterium smegmatis. Antimicrob. Agents Chemother. 2011 Apr 18.

2010

Borrelli S, Zandberg WF, Mohan S, Ko M, Martinez-Gutierrez F, Partha SK, Sanders DA, Av-Gay Y, Pinto BM. 2010. Antimycobacterial activity of UDP-galactopyranose mutase inhibitors. Int J Antimicrob Agents 36(4):364-8.

Capyk JK, Strynakda NC, and Eltis LD. 2010. 3-Ketosteroid-9a-Hydroxylase. In Handbook of Metalloproteins (Eds. Messerschmidt, Huber, Poulos, Wieghardt), John Wiley & Sons Ltd., Chichester, pp.632-642.

Chao JD, Papavinasasundaram KG, Zheng X, Chávez-Steenbock A, Wang X, Lee GQ, Av-Gay Y. 2010. Convergence of Ser/Thr and two-component signaling to coordinate expression of the dormancy regulon in Mycobacterium tuberculosis. J Biol Chem.285(38):29239-46.

Chao J, Wong D, Zheng X, Poirier V, Bach H, Hmama Z, Av-Gay Y. 2010.Protein kinase and phosphatase signaling in Mycobacterium tuberculosis physiology and pathogenesis. Biochim Biophys Acta. 1804(3):620-7.

Deshayes C, Bach H, Euphrasie D, Attarian R, Coureuil M, Sougakoff W, Laval F, Av-Gay Y, Daffé M, Etienne G, Reyrat JM. 2010. MmpS4 promotes glycopeptidolipids biosynthesis and export in Mycobacterium smegmatis. Mol Microbiol.78(4):989-1003.

Dresen C, Lin L, D’Angelo I, Tocheva EI, Strynadka N and Eltis LD. 2010. A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism. J Biol Chem. doi:10.1074/jbc.M109.09902

Lack N, Yam KC, Lowe ED, Horsman GP, Nobel R, Sim E, and Eltis LD. 2010. Characterization of a C-C hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism. J. Biol. Chem. J Biol. 285, 434-43.

Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, Ruiz F, Bach H, Av-Gay Y. 2010. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles.Nanomedicine Oct;6(5):681-8.

Mascarello A, Chiaradia LD, Vernal J, Villarino A, Guido RV, Perizzolo P, Poirier V, Wong D, Martins PG, Nunes RJ, Yunes RA, Andricopulo AD, Av-Gay Y, Terenzi H. Inhibition of Mycobacterium tuberculosis tyrosine phosphatase PtpA by synthetic chalcones: kinetics, molecular modeling, toxicity and effect on growth. Bioorg Med Chem. 18(11):3783-9.

Matieu J, Mohn WW, Eltis LD, LeBlanc JC, Stewart GR, Dresen C, Okamoto K, Alvarez PJJ. 2010. 7-Ketocholesterol Catabolism by Rhodococcus jostii RHA1. Appl. Environ. Microbiol. 76:352-355.

Movahedzadeh F, Wheeler PR, Dinadayala P, Av-Gay Y, Parish T, Daffé M, Stoker NG. 2010. Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis. BMC Microbiol. 10:50.

2009

Attarian R, Bennie C, Bach H, Av-Gay Y. 2009. G lutathione disulfide and S-nitrosoglutathione detoxification by Mycobacterium tuberculosis thioredoxin system. FEBS Lett. 2009 Sep 6. [Epub ahead of print]

Bach H, Wong D, Av-Gay Y. 2009. Mycobacterium tuberculosis PtkA is a novel protein tyrosine kinase whose substrate is PtpA. Biochem J. 420, 155-60.

Capyk JK, D’Angelo I, Strynadka N, and Eltis, L.. 2009. Characterization of 3-ketosteroid 9a-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis. J. Biol. Chem.284: 9937-9946.

Capyk JK, Kalscheuer R, Stewart GR, Liu J, Kwon H, Zhao R, Okamoto S, Jacobs WR Jr, Eltis LD and Mohn WW. 2009. Mycobacterial cytochrome P450 125 (Cyp125) catalyzes the terminal hydroxylation of C27-steroids. J Biol Chem. 284, 35534-42.

Chao J, Wong D, Zheng X, Poirier V, Bach H, Hmama Z, Av-Gay Y.2009. Protein kinase and phosphatase signaling in Mycobacterium tuberculosis physiology and pathogenesis.Biochim Biophys Acta. 2009 Sep 17. [Epub ahead of print]

Gopalaswamy R, Narayanan S, Chen B, Jacobs WR, Av-Gay Y. 2009. The serine/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection. FEMS Microbiol Lett. 295, 23-9.

Hickey TB, Thorson LM, Speert DP, Daffé M, Stokes RW. 2009. Mycobacterium tuberculosis Cpn60.2 and DnaK are located on the bacterial surface, where Cpn60.2 facilitates efficient bacterial association with macrophages. Infect Immun. 77, 3389-401.

Houben EN, Walburger A, Ferrari G, Nguyen L, Thompson CJ, Miess C, Vogel G, Mueller B, Pieters J. 2009. Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria. Mol Microbiol.72, 41-52.

Ramón-García S, Martín C, Thompson C.J., Aínsa J.A. 2009. Role of the Mycobacterium tuberculosis P55 efflux pump in intrinsic drug resistance, oxidative stress responses, and growth. Antimicrob Agents Chemother. 53, 3675-82.

Ramón-García, S., Ng C, Anderson, H, Westwell-Roper C, Chao J., Av-Gay Y, Roberge M, and Thompson CJ. 2009. Chemical inactivation of intrinsic drug resistances to enhance the activities of clinically approved antibiotics against Mycobacterium tuberculosis. Nature Biotechnology submitted.

Rosloniec K, Wilbrink M, Capyk JK, Mohn WW, Ostendorf M, van der Geize R, Dijkhuizen L, Eltis LD. 2009. Cytochrome P450 125 (CYP125) catalyzes C26-hydroxylation to initiate sterol side chain degradation. Mol Microbiol. 74: 1031-43. PMID: 19843222

Sun J, Deghmane AE, Bucci C, Hmama Z. 2009. Detection of activated Rab7 GTPase with an immobilized RILP probe.Methods Mol Biol. 2009;531:57-69.

Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z. 2009. A broad-range of recombination cloning vectors in mycobacteria.Plasmid. 2009 Jul 30. [Epub ahead of print]

Yam KC, D’Angelo I, Kalscheuer R, Zhu H, Wang J-X, Snieckus V, Jacobs Jr WR, S Strynadka N, and Eltis LD. 2009. Characterization of a cholesterol-degrading dioxygenase critical for pathogenecity of Mycobacterium tuberculosis. PLoS Pathog.5,e1000344.

Book chapters

Av-Gay, Y and Alber T. (2009). Prospects for TB therapeutics targeting Mycobacterium tuberculosis phosphosignaling networks. Protein Kinases as Drug Targets. Wiley VCH (15 Aug 2008). ISBN-13: 978-3527317905.

Review Article:

Chao, D. J., Wong, D., Zheng, X., Poirier, V., Bach, H., Hmama. Z., and Av-Gay, Y. Protein kinases and phosphatases signaling in Mycobacterium tuberculosis physiology and pathogenesis. Biochimica et Biophysica Acta – Proteins and Proteomics. In press.

2008

Bach, H. Papavinasasundaram, K. G., Wong , D., Hmama, Z., and Av-Gay, Y. 2008. Mycobacterium tuberculosis virulence is mediated by PtpA dephosphorylation of Human VPS33B. Cell: Host & Microbe 3(5):316-22

Dosanjh M, Newton GL, Davies J. 2008. Characterisation of a mycothiol ligase mutant of Rhodococcus jostii RHA1. Res Microbiol. 159: 643-50.

Gopalasawamy, R., Naryanan, S., Jacobs. W.R., Jr., Ko, M., and Av-Gay, Y. 2008. Protein kinase MSMEG3682 from M. smegmatis is essential for maintenance of cell wall architecture and cell division. FEMS Microbiology Letters. 278:121-127.

Lack N, Lowe ED, Liu J, Eltis LD, Noble MEM, Sim E, and Westwood IM. 2008. Structure of HsaD, a steroid-degrading hydrolase from Mycobacterium tuberculosis. Acta Crystallographica Section F. 64(Pt 1), 2-7.

Li AH, Lam WL, Stokes RW. 2008 Bacterial artificial chromosome fingerprint arrays for the differentiation of transcriptomic differences in mycobacteria. J Microbiol Methods 75, 416-24.

Li, A.H., Lam, W.L., Stokes, R.W. 2008. Characterization of genes differentially expressed within macrophages by virulent and attenuated Mycobacterium tuberculosis identifies candidate genes involved in intracellular growth. Microbiology. 154: 2291 – 2303.

Mohn WW, van der Geize R, Stewart GR, Okamoto S, Liu J, Dijkhuizen L, Eltis LD. 2008. The actinobacterial mce4 locus encodes a steroid transporter. J. Biol. Chem. 283(51):35368-35374

Narayan, H. L., Naryanan, S., Bach, H., Papavinasasundaram, K.G., and Av-Gay, Y. 2008. Molecular cloning and biochemical characterization of a serine threonine protein kinase, PknL, from Mycobacterium tuberculosis. Protein Expression and Purification. 58(2):309-17

Randhawa, A.K., Ziltener, H.J. and Stokes, R.W. 2008. CD43 controls intracellular growth of Mycobacterium tuberculosis through the induction of TNFa-mediated apoptosis. Cellular Microbiology. 10, 2105 – 2117.

Vilcheze, C., Av-Gay, Y. Attarian R,Liu Z, Hazbón MH, Colangeli R, Chen B, Liu W, Alland D, Sacchettini JC, Jacobs Jr WR. 2008. Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis. Molecular Microbiology. 69(5):1316-29.

Review Article

Hegymegi-Barakonyi B, Székely R, Varga Z, Kiss R, Borbély G, Németh G, Bánhegyi P, Pató J, Greff Z, Horváth Z, Mészáros G, Marosfalvi J, Er s D, Szántai-Kis C, Breza N, Garavaglia S, Perozzi S, Rizzi M, Hafenbradl D, Ko M, Av-Gay Y, Klebl BM, Orfi L, Kéri G. 2008. Signalling inhibitors against Mycobacterium tuberculosis -early days of a new therapeutic concept in tuberculosis. Curr Med Chem. 15, 2760-70.

2007

Deghmane AE, Soualhine H, Bach, H., Sendide K, Itoh S, Tam A, Noubir S, Talal A, Lo R, Toyoshima S, Av-Gay Y, Hmama Z. 2007. LpdC mediates retention of coronin-1 on M. bovis BCG vacuoles, leading to arrest in phagosome maturation. J. Cell Sci 120 (16):2796-2806

Lynett, J. and Stokes, R.W. 2007. Selection of transposon mutants of Mycobacterium tuberculosis with increased macrophage infectivity identifies fadD23 to be involved in sulfolipid production and association with macrophages. Microbiology. 153: 3133-3140.

Miller, C., Rawat, M., Johnson, T., and Av-Gay, Y. 2007. Innate Protection of Mycobacteria Against the Antimicrobial Activity of Nitric Oxide is Provided by Mycothiol. Anti Microbial Agents and Chemotherapy, 51(9):3364-3366.

Zheng, X., Papavinasasundaram, K.G., and Av-Gay, Y. 2007. Novel Substrates of Mycobacterium tuberculosis PknH serine / Threonine Kinase. BBRC.

Rawat, Mamta and and Yosef Av-Gay. 2007. Mycothiol dependent Proteins in Actinomycetes. FEMS Microbiology Reviews. 31:278-292.

Rawat, M., Johnson, T., Cadiz, V., and Av-Gay Y. 2007. Comparative analysis of mutants in the mycothiol biosynthesis pathway in Mycobacterium smegmatis. BBRC 363:71-76.

Soualhine H, Deghmane AE, Mak K, Amina Talal A, Av-Gay Y, Hmama Z. 2007. Recombinant Mycobacterium bovis BCG secreting cathepsin S promotes MHC class II-directed antigen presentation by the macrophage cell line THP-1. J Immunol . 179:5137-5145.

Sun J, Deghmane AE, Soualhine H, Hong T, Bucci C, Solodkin A, Hmama Z. 2007.
Mycobacterium bovis BCG disrupts the interaction of Rab7 with RILP contributing to inhibition of phagosome maturation. J Leukoc Biol. 82(6):1437-45.

Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Davies JE, Dijkhuizen L, Mohn WW, and Eltis LD. 2007. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Proc. Natl. Acad. Sci. USA 104, 1947-1952.

2006

Anderton MC, Bhakta S, Besra GS, Jeavons P, Eltis LD, and Sim E. 2006. Characterisation of the putative operon containing arylamine N-acetyltransferase (nat) in Mycobacterium bovis BCG. Mol. Microbiol. 59, 181-192.

Bach, H., Sun, J., Wong , D., Hmama, Z. and Av-Gay Y. 2006. Mycobacterium avium ssp paratuberculosis PtpA is an endogenous tyrosine phosphatase secreted during infection. Infection and Immunity. 4:6540-6546.

McLeod MM, Warren RL, Hsiao WWL, Araki N, Myhre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, Wang D, Dosanjh M, Hara H, Petrescu A, Morin RD, Yang G, Stott JM, Schein JE, Shin H, Smailus D, Siddiqui AS, Marra MA, Jones SJM, Holt R, Brinkman FSL, Miyauchi K, Fukuda M, Davies JE, Mohn WW, Eltis LD. 2006. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc Nat Acad Sci USA 103(42):15582-15587

Newton, G. L., Koledin, T., Ko, M.,Av-Gay, Y., and Fahey, R.C. 2006. N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-D-glucopyranoside Deacetylase,a Mycothiol Biosynthetic Enzyme (MshB), is a metalloprotein subject to feedback inhibition by Mycothiol. Protein Expression and Purification. 47:542-550.

Nguyen, L., and C. J. Thompson. 2006. Foundations of antibiotic resistance in bacterial physiology – the mycobacterial paradigm. Trends Microbiol. 14:304-312. PDF

Pérez, J., Garcia, R. L., Bach, H., H de Waard, J., Jacobs, W. R. Jr., Av-Gay, Y., Bubis, J and Takiff H. 2006. Mycobacterium tuberculosis transporter MmpL7 is a potential substrate for kinase PknD. BBRC. 348:6-12.

Pick, N., Rawat, M., Arad, D., Lan, J., Fan, J., Kende, A.S., and Av-Gay, Y. 2006. In Vitro Properties of Antimicrobial Bromo-Tyrosine Alkaloids. J. Med. Microbiol. 55: 407-415.

Ung S.E.K., and Av-Gay, Y. 2006. Mycothiol Dependent Mycobacterial Response to Oxidative Stress. FEBS Letters. 580:2712-2716.

2005

Morris, R. P., L. Nguyen, J. Gatfield, K. Visconti, K. T. Nguyen, D. Schnappinger, S. Ehrt, L. Heifets, J. Pieters, G. Schoolnik, and C. J. Thompson. 2005. Ancestral antibiotic resistance in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 102:12200-12205 PDF
(This work was recognized as a Research Highlight—“Antibiotic resistance: TB’s second secret”—by Nature, reviewed by Nature Chemical Biology and evaluated by Faculty of 1000 as a “Must Read”, rated 6.4)

Nguyen, L., S. Chinnapapagari, and C. J. Thompson. 2005. FbpA dependent biosynthesis of trehalose dimycolate is required for intrinsic multiple antibiotic resistance, cell wall structure and colonial morphology of Mycobacterium smegmatis. J. Bacteriol. 187:6603-6611. PDF

Randhawa, A.K., Ziltener, H.J., Merzaban, J.S. and Stokes, R.W. 2005. CD43 is required for optimal growth inhibition of Mycobacterium tuberculosis in macrophages and in mice. Journal of Immunology. 175: 1805-1812.

Rooyakkers, A.W.J. and Stokes, R.W. 2005. Absence of complement receptor 3 results in reduced binding and ingestion of Mycobacterium tuberculosis but has no significant effect on the induction of reactive oxygen and nitrogen intermediates or the survival of the bacteria in resident and interferon ? activated macrophages. Microbial Pathogenesis. 39: 57-67.

Prior to 2005

Dullaghan, EM, Malloff , CA , Li, AH, Lam, WL and Stokes, RW “Two-Dimensional Bacterial Genome Display: A Method For The Genomic Analysis Of Mycobacteria”. 2002. Microbiology 148: 3111-3117. * highlighted in “Hot of the Press” in Microbiology Today 29, 210 Nov 2002.

Hingley-Wilson SM, Sly LM, Reiner NE, McMaster WR. 2000. The immunobiology of the mycobacterial infected macrophage. Mod. Aspects of Immunobiol. 1:96-101.

López M, Sly LM, Luu Y, Young D, Cooper H, Reiner NE. 2003. The 19-kDa Mycobacterium tuberculosis protein induces macrophage apoptosis through Toll-like receptor-2. J Immunol. 170(5):2409-16.

Malloff, C.A, Dullaghan, E.M., Li, A,H., Stokes, R.W., Fernandez, R.C., and Lam, W.L. 2003. “ Two-dimensional DNA displays for comparisons of bacterial genomes. Biological Procedures Online 5 , 143-152.

Melo , MD , Catchpole, IR, Haggar, G. and Stokes, RW. 2000. Utilization of CD11b knockout mice to characterize the role of complement receptor 3 (CR3, CD11b/CD18) in the pathogenesis of Mycobacterium tuberculosis. Cellular Immunology 205: 13-23.

Melo, MD Stokes, RW. 2000. Interaction of Mycobacterium tuberculosis with MH-S, an immortalized murine alveolar macrophage cell line: a comparison with primary murine macrophages. Tubercle 80: 35-46.

Randhawa, A.K., Ziltener, H.J. and Stokes, R.W. 2003. Characterization of the interaction of macrophage CD43 with Mycobacterium tuberculosis. Canadian Journal of Clinical Pharmacology. 10: 99-100.

Sly LM, Hingley-Wilson SM, Reiner NE, McMaster WR. 2003. Survival of Mycobacterium tuberculosis in host macrophages involves resistance to apoptosis dependent upon induction of antiapoptotic Bcl-2 family member Mcl-1. J Immunol. 170(1):430-7.

Sly LM, Lopez M, Nauseef WN, Reiner NE. 2000. 1a,25-Dihydroxyvitamin D3-induced monocyte antimycobacterial activity is regulated by phosphatidylinositol 3-kinase and mediated by the NADPH-dependent phagocyte oxidase, J. Biol. Chem.276:35482-35493.

Stokes, R.W., Norris-Jones, R., Brooks, D.E., Beveridge, T.J. , Doxsee, D. and Thorson, L.M. 2004. The glycan rich outer layer of the cell wall envelope of Mycobacterium tuberculosis acts as an anti-phagocytic capsule limiting the association of the bacterium with macrophages. Infection and Immunity 72 : 5676-5686 (2004).

Thorson, LM, Doxsee, D, Scott, M, Wheeler, P and Stokes, RW. 2001. The effect of mycobacterial phospholipids on the interaction of Mycobacterium tuberculosis with macrophages. Infection and Immunity 69: 2172-2179 (2001).

Wang, J., Thorson, L., Stokes, R.W., Santosuosso, M., Huygen, K., Zganiacz, A., Hitt, M., and Xing, Z. 2004. Single Mucosal, but not Parenteral, Immunization with Recombinant Adenoviral-Based Vaccine Provides Potent Protection from Pulmonary Tuberculosis. Journal of Immunology 173: 6357-6365 (2004).